This detailed new edition combines a wide selection of experimental methods for plant cold acclimation, deacclimation, and cold memory, ranging from the whole plant level of ecology and breeding to molecular profiling and the detailed analysis of specific proteins, with many levels of investigated complexity in between. The collection updates methodology from the first edition while adding chapters on numerous key advances in the field. Written for the highly successful Method in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and up-to-date, Plant Cold Acclimation: Methods and Protocols, Second Edition seeks to aid researchers, both new and experienced, in entering this exciting field of research or broadening the scope of their investigations.
Inhaltsverzeichnis
Introduction: Plant Cold Acclimation and Winter Survival.- Measuring Freezing Tolerance of Leaves and Rosettes: Electrolyte Leakage and Chlorophyll Fluorescence Assays.- Differential Thermal Analysis: A Fast Alternative to Frost Tolerance Measurements.- Infrared Thermal Analysis of Plant Freezing Processes.- Conducting Field Trials for Frost Tolerance Breeding in Cereals.- A Whole-Plant Screening Test to Select Freezing-Tolerant and Low-Dormant Genotypes.- Mapping of Quantitative Trait Loci (QTL) Associated with Plant Freezing Tolerance and Cold Acclimation.- Identification of Arabidopsis Mutants with Altered Freezing Tolerance.- Cryo-Scanning Electron Microscopy to Study the Freezing Behavior of Plant Tissues.- Using Pixel-Based Microscope Images to Generate 3-D Reconstructions of Frozen and Thawed Plant Tissue.- Phenotyping Plant Cellular and Tissue Level Responses to Cold with Synchrotron-Based Fourier-Transform Infrared Spectroscopy and X-Ray Computed Tomography.- Proteomic Approaches to Identify Proteins Responsive to Cold Stress.- Proteomic Approaches to Identify Cold Regulated Plasma Membrane Proteins.- A Lipidomic Approach to Identify Cold-Induced Changes in Arabidopsis Membrane Lipid Composition.- Multiplexed Profiling and Data Processing Methods to Identify Temperature-Regulated Primary Metabolites Using Gas Chromatography Coupled to Mass Spectrometry.- Determining the ROS and the Antioxidant Status of Leaves during Cold Acclimation.- Analysis of Changes in Plant Cell Wall Composition and Structure during Cold Acclimation.- Subcellular Compartmentation of Metabolites Involved in Cold Acclimation.- Mathematical Modeling of Plant Metabolism in a Changing Temperature Regime.- Characterization of Ice Binding Proteins from Sea-Ice Microalgae.- Isolation and Characterization of Ice-Binding Proteins from Higher Plants.
