Cancer Immunotherapy

Isolation of live immune cells from the tumour microenvironment by FACS. -
In vitro
evaluation of cancer cell immunogenicity and antigen-specific T-cell cytotoxicity by flow cytometry. - Retroviral transduction of human primary T cells followed by real-time T-cell mediated cancer cell cytolysis analysis. - Expansion and retroviral transduction of primary murine T cells for CAR T cell therapy. - In Situ Decellularisation of Tissues applied to the topographical analysis of tumour associated extracellular matrix. - Monitoring cell cytoskeleton variations upon piezoelectric stimulation: Implications for the immune system. - Preparation method and
in vitro
characterization of nanoparticles sensitive to tumor microenvironment. - A new microfluidic device to facilitate functional precision medicine assays. - Kinetic detection of apoptosis events via caspase 3/7 activation in a tumor-immune microenvironment on a chip. - Hypoxic 3D Tumor Model for Evaluating of CAR-T Cell Therapy In Vitro. - Rapid screening of CAR T cell functional improvement strategies by highly-multiplexed single-cell secretomics. - Genome editing in CAR-T cells using CRISPR/Cas9 technology. - Genetic modification of tumor-infiltrating lymphocytes, peripheral T cells, and T cell model cell lines. - Transposon-based manufacturing of human CAR-T cells. - Redirecting human conventional and regulatory T cells using chimeric antigen receptors. - How to Test Human CAR T cells in Solid Tumors, The Next Frontier of CAR T Cell Therapy. - Nano-Optogenetic CAR-T Cell Immunotherapy. - A non-viral
piggyBac
transposon-mediated method to generate large-scale CAR-NK cells from human peripheral blood primary NK cells. - Engineering probiotic
E. coli
Nissle 1917 for release of therapeutic nanobodies.