Plant Cell Morphogenesis

This book collects techniques to continue exploring post-genomic land plant biology though the wisdom and skills accumulated from work on the founding molecular biology models that can now guide research into other species, including crop plants. Beginning with the visualization of plant cell structures, the volume moves on to cover digital image analysis protocols, qualitative and quantitative detection of the organization and dynamics of individual intracellular structures, the manipulation of intracellular structures, as well as techniques for studying model cell types. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and fully updated, Plant Cell Morphogenesis: Methods and Protocols, Second Edition serves as an ideal source of inspiration for further research into the morphogenesis of plant cells, tissues, and organs.

1. Essential Methods of Plant Sample Preparation for Light Microscopy.- 2. Selected Simple Methods of Plant Cell Wall Histochemistry and Staining for Light Microscopy.- 3. Chemical Fixation, Immunofluorescence, and Immunogold Labelling of Electron Microscopical Sections.- 4. Essential Methods of Plant Sample Preparation for High Resolution Scanning Electron Microscopy at Room Temperature.- 5. Fluorescence Lifetime Imaging of Plant Cell Walls.- 6. Raman Spectroscopy in Non-Woody Plants.- 7. Image Analysis: Basic Procedures for Description of Plant Structures.- 8. From Data to Illustrations: Common (Free) Tools for Proper Image Data Handling and Processing.- 9. Visualizing and Quantifying In Vivo Cortical Cytoskeleton Structure and Dynamics.- 10. Quantitative and Comparative Analysis of Global Patterns of (Microtubule) Cytoskeleton Organization with CytoskeletonAnalyzer2D.- 11. Using FM Dyes to Study Endomembranes and Their Dynamics in Plants and Cell Suspensions.- 12. Transient GeneExpression as a Tool to Monitor and Manipulate the Levels of Acidic Phospholipids in Plant Cells.- 13. The Photo-Convertible Fluorescent Protein Dendra2 Tag as a Tool to Investigate Intracellular Protein Dynamics.- 14. Cellular Force Microscopy to Measure Mechanical Forces in Plant Cells.- 15. Optical Trapping in Plant Cells.- 16. Sequential Replicas: Method for In Vivo Imaging of Plant Organ Surfaces that Undergo Deformation.- 17. Time-Lapse Imaging of Developing Shoot Meristems Using Confocal Laser Scanning Microscope.- 18. Quantifying Plant Growth and Cell Proliferation with MorphoGraphX.- 19. Kinematic Characterization of Root Growth by Means of Stripflow.- 20. Automated Acquisition and Morphological Analysis of Cell Growth Mutants in
Physcomitrella patens.-
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Live Cell Imaging of Arabidopsis Root Hairs.- 22. Morphological Analysis of Leaf Epidermis Pavement Cells with PaCeQuant.- 23. Imaging of Developing Metaxylem Vessel Elements in Cultured Hypocotyls.- 24.Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes.- 25. Plant Cell Lines in Cell Morphogenesis Research: From Phenotyping to Omics.