Protides of the Biological Fluids

Protides of the Biological Fluids presents the functional approach to conformation that combines the physiological significance with physical and chemical appearance of the protein. This book discusses the technical details of purification, determination, or identification of given protein fractions.
Organized into three sections, this book begins with an overview of the intramolecular cross-linked synthetic polypeptides that can be used as models for the organized spatial structure of proteins. This text then examines the polarization of fluorescence methods, which provide very sensitive hydrodynamic techniques for studying the internal organization and the rigidity of the polypeptide. Other sections consider the excretion of different classes of immunoglobulins in the external secretions of dogs. This book discusses as well the anti-coli antibody and the poliovirus neutralizing antibody activity of achlorhydric gastric juices. The final section deals with the introduction of gels as a medium for the electrophoresis of proteins.
This book is a valuable resource for chemists, biochemists, and geneticists.

1;Front Cover ;1 2;Protides of the Biological Fluids;4 3;Copyright;5 4;Table of Contents;6 5;Preface;14 6;Acknowledgements;16 7;ACADEMIC LECTURE Analyses Immunochimiques des Tissus;18 8;SECTION A: CONFORMATION AND STRUCTURE OF PROTEINS;36 8.1;A1. Protein Models;38 8.1.1;Polypeptides as Models for the Tertiary Structure of Proteins;38 8.1.2;ß-Lactoglobulin as a Model of Subunit Enzymes;50 8.1.3;Preferential Binding of Solvent Components to Protein in Mixed WaterOrganic Solvent Systems;58 8.1.4;Some Aspects of Protein Models and Biological Function;68 8.1.5;Uses and Limitations of Various Types of Atomic Models in Molecular Representation;82 8.1.6;Chemical, Morphological and Immunologic Properties of Recombined Red Cell Membranes;88 8.2;A2. Conformation and Dielectric Behaviour;98 8.2.1;Messungen der Dielektrizitätskonstanten von Hämoglobinstrukturänderungen in Wässriger Lösung mit einem Mikrowelleninterfer...;98 8.2.2;R. F. Measurements of the Dielectric Properties of Strongly Conducting Liquids;104 8.2.3;Size and Shape Determination of Globular Proteins I. Bridge and Cell Problems in the Dielectric Measurement of Protein Solut...;112 8.2.4;Size and Shape Determination of Globular Proteins. II. Dielectric Relaxation and Viscosity Determination of Globular Proteins;118 8.2.5;The Dipole Moment in Sperm Whale Myoglobin;126 8.3;A3. Structure by Optical Methods;132 8.3.1;Application de la Diffusion de la Lumière à l'Étude de quelques Macromolécules Biologiques;132 8.3.2;Optical Rotation Studies on Globular Serum Proteins and Isolated Polypeptide Chains of Immunoglobulins;144 8.3.3;On the ß-Conformation in Eye-lens Proteins;154 8.3.4;Messung der Optischen Rotationsdispersion von Nativer und Modifizierter Lactatdehydrogenase sowie des EnzymCoenzym-Kompl...;160 8.3.5;Circular Dichroism of the ß-Lactoglobulins between 190 and 320 nm;166 8.4;A4. Structure by Other Methods;172 8.4.1;Elektronenmikroskopische Untersuchung zur Struktur von Fibrinfibrillen;172 8.4.2;Study of the Al
kaline Bohr Effect of Hemoglobin by means of Differential Hydrogen Ion Titration Curves;178 8.4.3;Kinetic Studies of AntibodyHapten Reactions with the Temperature-jump Relaxation Technique;184 8.5;A5. Structure of Lysozyme;190 8.5.1;The Structure and Function of Lysozyme;190 8.5.2;Relations entre Structure et Activité chez quelques Lysozymes de Blancs d'eufs d'Oiseaux et d'Origine Humaine;198 8.5.3;Constantes Apparentes d'Affinité de Lysozymes d'Origines Diverses pour Micrococcus lysodeikticus;208 8.5.4;Immunochemical Analyses of Human Lysozyme in Monocytic Dyscrasias;214 8.6;A6. Structure of Immunoglobulins;222 8.6.1;Structure and Cellular Localization of Secretory IgA;222 8.6.2;The Secretory Immunologic System;238 8.6.3;Les Fragments de Protéolyse Enzymatique des IgM Globulines Humaines;244 8.6.4;Rapports des Équivalents Structuraux de la Gamma G et de la Gamma A vis-à-vis de Certaines Activités Biologiques;250 8.7;A7. Structure of Various Proteins;258 8.7.1;Nature and Functions of Mucins and Blood Group Substances;258 8.7.2;Dissociation of Human a2-Macroglobulin at Acid and Alkaline pH;264 8.7.3;Structure Quaternaire des Alpha-2-macroglobulines Humaines Normales;272 8.7.4;Untersuchungen zur Lokalisation der Insertionsstellen Insulinbindender Antikörper am Insulinmolekül;282 9;SECTION B: PROTEINS OF BODILY SECRETIONS;290 9.1;B1. General Papers;292 9.1.1;Studies of One Specialized Local Antibody System;292 9.1.2;Studies on Immunoglobulins of Secretions in Dogs and Mice;300 9.1.3;The Fundamental Structure of the Glycoproteins in Mucous Secretions;304 9.1.4;The Compositional Pattern of the Carbohydrates of Glycoproteins of Epithelial Secretions;310 9.1.5;The Composition of Mucus with Special Reference to its Rheological Properties;316 9.2;B2. Nasal Secretions;324 9.2.1;Factors Influencing the Production of Secretory IgA Antibody in Nasal Secretions;324 9.2.2;The Biologic Significance of IgA in the Mucous Secretions: Response to Oral Polio Vaccine in IgA-deficient Sub
jects;332 9.2.3;Localization of External Secretory IgA Piece by Immunofluorescence in Tissues from Patients with Agammaglobulinemia;342 9.2.4;Local Synthesis of Immunoglobulin G in the Upper Respiratory Tract;348 9.2.5;Protein and Lipid Pattern in Serous Otitis Media;354 9.3;B3. Bronchial Secretions;360 9.3.1;Biochemical Exploration of Bronchial Hypersecretions;360 9.3.2;Etude des Glycopeptides du Mucus Fibrillaire de la Sécrétion Bronchique;378 9.3.3;Secretions of Proteins into the Sputum in Chronic Bronchitis;388 9.4;B4. Gastro-Intestinal SecretionsSaliva;396 9.4.1;Mucous in Normal Human Submaxillary Saliva;396 9.4.2;Der Nachweis von M-Komponenten der Immunglobuline im Speichel von Patienten mit Paraproteinämien;402 9.4.3;The Relation between the Histology of the Gastric Mucosa, Secretion of Intrinsic Factor, Autoimmunity and the Absorption o...;410 9.4.4;The Vitamin B12, Transport Proteins in Body Fluids and Leucocytes;418 9.4.5;Etude de Deux Transporteurs de Vitamine B12, Isolés de la Muqueuse Gastrique de Porc;426 9.4.6;Autoimmunization to Intrinsic Factor and Immunization to Oral Hog Intrinsic Factor in ManSome Characteristic Features;440 9.4.7;Rôle des Glycoprotéines Sulfatées dans les Méchanismes de Protection de la Muqueuse Gastrique;446 9.4.8;Antibody Content of Anacid Gastric Juices: The occurrence of E. coli Antibodies and Poliovirus Neutralizing Antibodies;452 9.4.9;Über die Isolierung und Immunchemische Charakterisierung der Antigenen Magensaftesterase VI A;458 9.4.10;Isolement et Caractérisation de Chondro.tine-4-Sulfate dans la Sécrétion Gastrique Fundique chez le Chien;468 9.4.11;Coproantibody and Defense Mechanisms of the Intestinal Mucosal Surface;476 9.4.12;Immunoglobulin Production and Secretion into the Intestinal Tract of Primates under the Influence of Some Antigenic Stimuli;486 9.4.13;Immunoglobulin Synthesis by Tissues of the Gastro-intestinal and Respiratory Tracts;496 9.4.14;Increased Serum IgA in Intestinal Disease;502 9.4.15;Source of I
gA in Jejunal Secretions;508 9.4.16;Protein Synthesis by the Human Exocrine Pancreas;516 9.4.17;Présence d'une Protéine d'Origine Tissulaire, l's2H dans le Sérum de Sujets atteints d'Affections Malignes;528 9.4.18;First Meconium in the Detection of Fibrocystic Disease of the Pancreas;534 9.5;B5. Genital Secretions;542 9.5.1;Muramidase (Lysozyme) in Cervical Secretions;542 9.5.2;Enzymes and Proteins in Uterine Mucus;552 9.5.3;A Seminal Fluid Component used for Desensibilization of Women with Unexplained Infertility;558 9.5.4;The Antigens of Human Seminal Plasma (with Special Reference to Lactoferrin as a Spermatozoa-coating Antigen);566 9.5.5;Purification of the 3.72 S Protein of the Human Seminal Fluid by Fractionation on Sephadex G 200 and Preparative Agar Gel Electrophoresis;570 9.6;B6. Urinary Proteins;576 9.6.1;"Tissue" Antigens and Enzymes in Human Urine;576 9.6.2;Caractérisation et Séparation du Pepsinogène Urinair: Corrélations avec la Sécrétion Gastrique;590 9.6.3;Polymorphisme Immunologique des Molécules de .G Excrétées au cours de Protéinuries;602 9.6.4;Les Facteurs Gm, Inv et ISf des Urines Normales;610 9.6.5;Nachweis Elektrophoretisch Aufgetrennter Harnkolloide durch Kaliumpermanganat;614 9.6.6;The Level of Plasma Proteins in Normal Human Urine;620 9.6.7;Les Mucopolysaccharides Urinaires dans l'Epilepsie Myoclonique Progressive Familiale (types Lafora et UnverrichtLundborg);628 9.7;B7. Milk Proteins;636 9.7.1;Ekkrinosiderophilin of Human Milk;636 9.7.2;The Role of Lactoferrin in Ion Absorption;644 9.7.3;New Data on Lactoferrin, the Iron-binding Protein of Secretions;650 9.7.4;The Origin of Immunoglobulin in Ruminant Milk;656 9.7.5;Isolement et Caractérisation de l' Auto-antigène, Responsable de la Formation d'Auto-anticorps chez les Malades Atteints de...;664 9.7.6;Comparison of Rabbit ImmunoglobulinsColostral IgA;676 9.7.7;Preferential Adsorption of a Single Bovine IgG Type by Isolated Epithelial Cells of the Mammary Gland;680 9.8;B8. Extra-cellular Flu
id Proteins;686 9.8.1;Les Immunoglobulines Seriques IgG, IgA et IgM dans les Liquides Ascitiques de Nature Différente;686 9.8.2;Extravascular Plasma Albumin in the Kidney and Its Possible Role in Urine Formation;690 9.8.3;Les Antigènes du Liquide Amniotique Humain;696 9.8.4;The Transcapillary Passage of Different Proteins in the Operative Wound;702 10;SECTION C. TECHNIQUES;710 10.1;C1. Electro-focusing;712 10.1.1;Isoelectric Focusing of an Estradiol Bindings ß-Globulin Present in Human Serum;712 10.1.2;Electro-focusing Studies in the Protein HormonesHuman Chorionic Gonadotrophin, Human Luteinizing Hormone and Pregnant Ma...;718 10.1.3;The Subunits of Low- and High-density Lipoproteins;724 10.2;C2. Determination of Fractions;732 10.2.1;The Stokes Radius of Macromolecules Determined from Electrophoresis in Agarose;732 10.2.2;Lipoprotein Electrophoresis on Gelatinized Cellulose Acetate;740 10.2.3;Free Ammo Acids of the Blood Plasma in Cases of Megaloblastic Anaemia;748 10.2.4;The Separation and Quantitative Analysis of Gamma-aminobutyric Acid (Gaba) by One-dimensional Paper Chromatography;752 10.2.5;Isolation of Components of Fragmented Normal Human IgG Immunoglobulin;758 10.2.6;A Longitudinal Study of the Serum Ceruloplasmin, a2-Macroglobulin, Transferrin and Immunoglobulins IgA, IgG and IgM in Chi...;764 10.2.7;The Turnover in Humans of Polymerized Human Serum Albumin and of Fractions of Human Serum Albumin with Different NF Trans...;772 10.2.8;Variations in Protein Synthesis Rate Demonstrated with the Fluorescent Antibody Technique;780 11;INDEX;786