Metabolomics

This second edition volume brings together some of the best experts in the field of modern metabolomics to discuss the latest various techniques used to study specific metabolite classes, and metabolomics in bacterial and mammalian systems. The chapters in this book cover topics such as handling big data for metabolite identification and quantification, as well as building pathways for comparison with other omes, Isotopic Ratio Outlier Analysis (IROA) for quantitative analysis; cholesterol and derivatives in ocular tissues using LC-MS/MS methods; microbial metabolites analysis by mass spectrometry; the metabolomic study of tissues in different diseases; and NMR analysis in livestock metabolomics. It also includes several chapters on the emerging area of spatial metabolomics. Written in the highly successful Methods in Molecular Biology series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding pitfalls.

Cutting-edge and authoritative, Metabolomics: Methods and Protocols, Second Edition is a valuable resource for any researcher looking to expand their knowledge about this important and advancing field.

Non-Targeted Metabolomics using the Sciex ZenoTOF 7600. - Analysis of Soybean Phospholipid Metabolites Using Triple Quadrupole Mass Spectrometry. - Magnetic Resonance Applications to Metabolism. - Isotopic Ratio Outlier Analysis (IROA) for Quantitative Analysis. - Chromatographic Choice in Cerebrospinal Fluid Metabolomics Identification Outcome. - Metabolite Analysis from Protein Deposits. - Comparison of Untargeted LC-MS/MS Metabolomics Data Processing Through Compound Discoverer and MS-DIAL. - Pathway Analysis Utilizing Metabolomic and Proteomic Datasets. - Metabolite Analog Induced Proteome Changes. - Spatial Atmospheric Pressure MALDI HRMS Analysis of Histological Sections of FFPE Samples Reveals a Unique Subset of the Metabolome. - Polyamine Metabolism in Optic Nerve: Spatial Metabolomics with AP/MALDI Mass Spectrometry Imaging. - Direct Colony Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Assay for Targeted Operon Metabolite Analysis. - Metabolomics of the Kynurenine Pathway by Laser Desorption Ionization Mass Spectrometry (LDI-MS). - Metabolic Pathway Tracing for NAD+ Synthesis and Consumption. - LC-MS-Based Metabolic Fingerprinting of Vitreous Humor. - Separation and Quantification of Isomeric Forms of Aminobutyric Acids. - Analytical Techniques for Melanin Identification and Estimation by Mass Spectrometry. - Quantification of Melatonin with Immunoassay and Liquid Chromatography-Tandem Mass Spectrometry. - Preparation of Human Trabecular Meshwork Cells for Metabolomic Studies. - Revealing the Metabolome: Metabolite Workflow for Retinal Ganglion Cells. - Protocol to Use Mouse Hepatocyte Cell Line AML12 to Study Hepatic Metabolism In Vitro. - Untargeted Metabolomics of Human Embryonic Kidney (HEK) Cell Line. - Reproducible Sectioning and Sample Preparation for Mass Spectrometry Imaging of Cancer Metabolism in Frozen Lung Tissue. - Protocols for Metabolomic Analyses to Assess Changes in Central Carbon Metabolism from Neural Tissue. - NMR Metabolomics in Livestock: An Update of Current Methods and Applications. - Untargeted Metabolomic Analysis in the Short-Lived Killifish using HILIC Ultra Performance Liquid Chromatography Method Coupled to a Q Exactive Orbitrap Mass Spectrometer. - Bioinformatic Analysis of Alzheimer s Disease Metabolomic Datasets for Ceramides and Gangliosides. - A Synthetic Nanoparticle to Mimic High-Density Lipoprotein for Reverse Cholesterol Transport.